It is increasingly important to cultivate novel deep-sea microorganisms in an effort to qualify the hypothesized metabolic potential from recent deep ocean environmental genomic studies. While the genomic data will inform cultivation efforts, sequence information must be placed in the context of the physiology of the organisms and their contributions to the ecology of their communities as well as to relevant biogeochemical processes. We are currently developing high-throughput cultivation techniques (see schematic below) as well as using traditional batch enrichments at high pressure to obtain new psychropiezophiles from novel phylogenetic lineages.