9129767
XBFFRTV5
1
apa
50
date
desc
year
Bartlett
52
https://bartlettlab.ucsd.edu/wp-content/plugins/zotpress/
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22%2C%22firstName%22%3A%22J.%20A.%22%2C%22lastName%22%3A%22Ugalde%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22K.%20K.%22%2C%22lastName%22%3A%22Mullane%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R.%20A.%22%2C%22lastName%22%3A%22Chastain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%20A.%22%2C%22lastName%22%3A%22Yayanos%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Kusube%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B.%20A.%22%2C%22lastName%22%3A%22Methe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%20H.%22%2C%22lastName%22%3A%22Bartlett%22%7D%5D%2C%22abstractNote%22%3A%22BackgroundThe%20deep%20ocean%20is%20characterized%20by%20low%20temperatures%2C%20high%20hydrostatic%20pressures%2C%20and%20low%20concentrations%20of%20organic%20matter.%20While%20these%20conditions%20likely%20select%20for%20distinct%20genomic%20characteristics%20within%20prokaryotes%2C%20the%20attributes%20facilitating%20adaptation%20to%20the%20deep%20ocean%20are%20relatively%20unexplored.%20In%20this%20study%2C%20we%20compared%20the%20genomes%20of%20seven%20strains%20within%20the%20genus%20Colwellia%2C%20including%20some%20of%20the%20most%20piezophilic%20microbes%20known%2C%20to%20identify%20genomic%20features%20that%20enable%20life%20in%20the%20deep%20sea.ResultsSignificant%20differences%20were%20found%20to%20exist%20between%20piezophilic%20and%20non-piezophilic%20strains%20of%20Colwellia.%20Piezophilic%20Colwellia%20have%20a%20more%20basic%20and%20hydrophobic%20proteome.%20The%20piezophilic%20abyssal%20and%20hadal%20isolates%20have%20more%20genes%20involved%20in%20replication%5C%2Frecombination%5C%2Frepair%2C%20cell%20wall%5C%2Fmembrane%20biogenesis%2C%20and%20cell%20motility.%20The%20characteristics%20of%20respiration%2C%20pilus%20generation%2C%20and%20membrane%20fluidity%20adjustment%20vary%20between%20the%20strains%2C%20with%20operons%20for%20a%20nuo%20dehydrogenase%20and%20a%20tad%20pilus%20only%20present%20in%20the%20piezophiles.%20In%20contrast%2C%20the%20piezosensitive%20members%20are%20unique%20in%20having%20the%20capacity%20for%20dissimilatory%20nitrite%20and%20TMAO%20reduction.%20A%20number%20of%20genes%20exist%20only%20within%20deep-sea%20adapted%20species%2C%20such%20as%20those%20encoding%20d-alanine-d-alanine%20ligase%20for%20peptidoglycan%20formation%2C%20alanine%20dehydrogenase%20for%20NADH%5C%2FNAD%28%2B%29%20homeostasis%2C%20and%20a%20SAM%20methyltransferase%20for%20tRNA%20modification.%20Many%20of%20these%20piezophile-specific%20genes%20are%20in%20variable%20regions%20of%20the%20genome%20near%20genomic%20islands%2C%20transposases%2C%20and%20toxin-antitoxin%20systems.ConclusionsWe%20identified%20a%20number%20of%20adaptations%20that%20may%20facilitate%20deep-sea%20radiation%20in%20members%20of%20the%20genus%20Colwellia%2C%20as%20well%20as%20in%20other%20piezophilic%20bacteria.%20An%20enrichment%20in%20more%20basic%20and%20hydrophobic%20amino%20acids%20could%20help%20piezophiles%20stabilize%20and%20limit%20water%20intrusion%20into%20proteins%20as%20a%20result%20of%20high%20pressure.%20Variations%20in%20genes%20associated%20with%20the%20membrane%2C%20including%20those%20involved%20in%20unsaturated%20fatty%20acid%20production%20and%20respiration%2C%20indicate%20that%20membrane-based%20adaptations%20are%20critical%20for%20coping%20with%20high%20pressure.%20The%20presence%20of%20many%20piezophile-specific%20genes%20near%20genomic%20islands%20highlights%20that%20adaptation%20to%20the%20deep%20ocean%20may%20be%20facilitated%20by%20horizontal%20gene%20transfer%20through%20transposases%20or%20other%20mobile%20elements.%20Some%20of%20these%20genes%20are%20amenable%20to%20further%20study%20in%20genetically%20tractable%20piezophilic%20and%20piezotolerant%20deep-sea%20microorganisms.%22%2C%22date%22%3A%222020%5C%2F10%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1186%5C%2Fs12864-020-07102-y%22%2C%22ISSN%22%3A%221471-2164%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22XBFFRTV5%22%5D%2C%22dateModified%22%3A%222022-05-25T23%3A20%3A04Z%22%7D%7D%2C%7B%22key%22%3A%22PE264PJR%22%2C%22library%22%3A%7B%22id%22%3A9129767%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hand%20et%20al.%22%2C%22parsedDate%22%3A%222020-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BHand%2C%20K.%20P.%2C%20%26lt%3Bstrong%26gt%3BBartlett%26lt%3B%5C%2Fstrong%26gt%3B%2C%20D.%20H.%2C%20Fryer%2C%20P.%2C%20Peoples%2C%20L.%2C%20Williford%2C%20K.%2C%20Hofmann%2C%20A.%20E.%2C%20%26amp%3B%20Cameron%2C%20J.%20%282020%29.%20Discovery%20of%20novel%20structures%20at%2010.7%20km%20depth%20in%20the%20Mariana%20Trench%20may%20reveal%20chemolithoautotrophic%20microbial%20communities.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